Replacing animal-derived reagents, simulating in utero microinjections and clicker training for mouse gait assessment

Assessing enrichment, consolidating animal research guidance and understanding mouse aggression

February 2023   The papers behind the pod: 1. Hobbiesiefken U et al. (2023). Rating enrichment items by female group-housed laboratory mice in multiple binary choice tests using an RFID-based tracking system. PloS one 18(1): e0278709. doi: 10.1371/journal.pone.0278709 [https://doi.org/10.1371%2Fjournal.pone.0278709] 2. Petkov C et al. (2022). Unified ethical principles and an animal research ‘Helsinki’ declaration as foundations for international collaboration. Current Research in Neurobiology 3:100060. doi: 10.1016/j.crneur.2022.100060 [https://doi.org/10.1016/j.crneur.2022.100060] 3. Weber E et al. (2023). Aggression in Group-Housed Male Mice: A Systematic Review. Animals 13(1):143. doi: 10.3390/ani13010143 [https://doi.org/10.3390%2Fani13010143] It’s the third Thursday of February and you’re listening to the final episode of 3 minute 3Rs, recapping the latest efforts to replace, reduce and refine the use of animals in research. Follow this link for the full transcript: https://www.nc3rs.org.uk/3-minute-3rs-podcast-february-2023-transcript [https://www.nc3rs.org.uk/3-minute-3rs-podcast-february-2023-transcript] ---------------------------------------- Hosted on Acast. See acast.com/privacy [https://acast.com/privacy] for more information.

Replacing animal-derived reagents, simulating in utero microinjections and clicker training for mouse gait assessment

January 2023   The papers behind the pod: 1. Cassotta M et al. (2022). A worldwide survey on the use of animal-derived materials and reagents in scientific experimentation. Engineering in Life Sciences 22(9):561-604. doi: 10.1002/elsc.202100167 [https://onlinelibrary.wiley.com/doi/epdf/10.1002/elsc.202100167]   2. Nuber M et al. (2022). Development of a 3D simulator for training the mouse in utero electroporation. PLOS One 17(12): e0279004. doi: 10.1371/journal.pone.0279004 [https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0279004] 3. Dickmann J et al. (2022). Clicker Training Mice for Improved Compliance in the Catwalk Test. Animals 12(24): 3545. doi: 10.3390/ani12243545 [https://doi.org/10.3390%2Fani12243545] Happy New Year to all 3 Minute 3Rs listeners. It’s 2023, and we’re back with three more papers highlighting efforts to replace, reduce and refine the use of animals in research. Follow this link for the full transcript: https://www.nc3rs.org.uk/3-minute-3rs-podcast-january-2023-transcript   ---------------------------------------- Hosted on Acast. See acast.com/privacy [https://acast.com/privacy] for more information.

Kidney organoid vascularisation, implementing masking and quantitatively assessing experiment severity

December 2022 The papers behind the pod: 1. Menéndez ABC et al. (2022). Creating a kidney organoid-vasculature interaction model using a novel organ-on-chip system. Scientific Reports 12:20699. doi: 10.1038/s41598-022-24945-5 [https://doi.org/10.1038/s41598-022-24945-5] 2. Karp N et al. (2022). A qualitative study of the barriers to using blinding in in vivo experiments and suggestions for improvement. PLOS Biology 20(11): e3001873. doi: 10.1371/journal.pbio.3001873 [https://doi.org/10.1371/journal.pbio.3001873] 3. Talbot S et al. (2022). RELSA—A multidimensional procedure for the comparative assessment of well-being and the quantitative determination of severity in experimental procedures. Frontiers in Veterinary Science 9:937711. doi: 10.3389/fvets.2022.937711 [https://doi.org/10.3389/fvets.2022.937711]   It’s the third Thursday of December, and you’re listening to 3 Minute 3Rs, your monthly recap of efforts to replace, reduce and refine the use of animals in research. To round off 2022 we are highlighting a paper for each R. Follow this link for the full transcript: https://www.nc3rs.org.uk/3-minute-3rs-podcast-december-2022-transcript ---------------------------------------- Hosted on Acast. See acast.com/privacy [https://acast.com/privacy] for more information.

Assessing pain, living systematic reviews and inducing focal hypoxia in human neurons

November 2022 The papers behind the pod: 1. Aulehner K et al. (2022). Grimace scale, burrowing, and nest building for the assessment of post-surgical pain in mice and rats—A systematic review. Frontiers in Veterinary Science 9:930005. doi: 10.3389/fvets.2022.930005 [https://www.frontiersin.org/articles/10.3389/fvets.2022.930005/full] 2. Hair K et al. (2022). ‘Living’ evidence frameworks for in vivo animal research: towards translational evidence-based medicine. BMJ Evidence-Based Medicine 2022;27:A17. doi: 10.1136/ebm-2022-EBMLive.31 [http://dx.doi.org/10.1136/ebm-2022-EBMLive.31] 3. Wong J et al. (2022). Electrochemically induced in vitro focal hypoxia in human neurons. Frontiers in Cell and Developmental Biology 10:968341. doi: 10.3389/fcell.2022.968341 [https://doi.org/10.3389%2Ffcell.2022.968341] It’s the third Thursday of November, and you’re listening to 3 Minute 3Rs, your monthly recap of efforts to replace, reduce and refine the use of animals in research. Follow this link for the full transcript: https://www.nc3rs.org.uk/3-minute-3rs-podcast-november-2022-transcript [https://www.nc3rs.org.uk/3-minute-3rs-podcast-november-2022-transcript] ---------------------------------------- Hosted on Acast. See acast.com/privacy [https://acast.com/privacy] for more information.

Statistical planning, human cell cultures for toxoplasma and preventing boredom in laboratory rodents

October 2022 The papers behind the pod: 1. Piper, SK et al. (2022). Statistical review of animal trials—A guideline. Biometrical Journal https://doi.org/10.1002/bimj.202200061 [https://doi.org/10.1002/bimj.202200061] 2. Gargaté MJ et al. (2022). Parallel Propagation of Toxoplasma gondii In Vivo, In Vitro and in Alternate Model: Towards Less Dependence on the Mice Model. Pathogens https://doi.org/10.3390/pathogens11091038 [https://doi.org/10.3390/pathogens11091038] 3. Mieske P et al. et al. (2022). Bored at home?—A systematic review on the effect of environmental enrichment on the welfare of laboratory rats and mice. Frontiers in Veterinary Science https://doi.org/10.3389/fvets.2022.899219 [https://doi.org/10.3389/fvets.2022.899219] It’s the third Thursday of October, and you’re listening to 3 Minute 3Rs, your monthly recap of efforts to replace, reduce and refine the use of animals in research. This month we have a paper on each R. Follow this link for the full transcript: https://nc3rs.org.uk/3-minute-3rs-podcast-october-2022-transcript [https://nc3rs.org.uk/3-minute-3rs-podcast-october-2022-transcript] ---------------------------------------- Hosted on Acast. See acast.com/privacy [https://acast.com/privacy] for more information.