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Many animals hibernate, but only the wood frog can freeze solid in winter—its heart and brain stopping completely—then thaw and come back to life in the spring. Its process to do this is fascinating … and could one day save human lives. The danger with freezing is ice. If water freezes within a cell, sharp ice crystals will puncture or rupture cell walls and kill the cell, which normally kills the animal. So, as temperatures drop, the wood frog’s liver converts glycogen to glucose, a sugar syrup, and floods the frog’s system. Meanwhile, nucleating proteins push water from the frog’s cells. Glucose replaces the water, which keeps the cells from freezing. Outside the cells, the water does freeze, which stops the frog’s metabolism, including its brain and heart, and preserves its tissues. In spring, the frog thaws, its brain and organs come back to life, and breeding season begins. Several other animals use chemical antifreezes to avoid ice crystals forming within their cells. And now researchers are trying to do the same with human organs harvested for transplant. Organs currently remain viable for only a few hours outside the body. But if glucose could replace the water within them, perhaps they too could be frozen solid and later reanimated, greatly prolonging transplant time. A potentially life-saving technique for humans, learned from the humble wood frog.
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